Cells of the zona fasciculata and zona reticularis lack aldosterone synthase (CYP11B2) that converts corticosterone to aldosterone, and thus these tissues produce only the weak mineralocorticoid corticosterone. However, both these zones do contain the CYP17A1 missing in zona glomerulosa and thus produce the major glucocorticoid, cortisol. Zona fasciculata and zona reticularis cells also contain CYP17A1, whose 17,20-lyase activity is responsible for producing the androgens, dehydroepiandrosterone (DHEA) and androstenedione. Thus, fasciculata and reticularis cells can make corticosteroids and the adrenal androgens, but not aldosterone.
This poster evaluates 3 different sample preparation approaches (ISOLUTE SLE+, EVOLUTE EXPRESS ABN, EVOLUTE EXPRESS CX) for extraction of large multi-drug urine panels.
Each approach is assessed in terms of suitability for extraction of analytes with different different properties (pka, LogP etc).
Tags: 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH) , 6-acetylmorphine (MAM) , 7-Aminoclonazepam , 96-well plate , Alprazolam , Amitriptyline , Amphetamine , Amphetamines , Analgesics , Barbiturates , Benzodiazepines , Benzoylecgonine , Buprenorphine , Butalbital , Cannabinoid , Carbamate hypnotic , Carbamate muscle relaxant , Carisoprodol , Chlordiazepoxide , Clinical , Clonazepam , Cocaine , Cocaines , Codeine , Diazepam , Dihydrocodeine (DHC) , Drugs of Abuse , EDDP , English , Fentanyl , Forensic , Gabapentin , Hydrocodone , Hydrolysed urine , Hydromorphone , Ketamine , LC-MS/MS , Lorazepam , MDMA , Meperidine , Meprobamate , Methadone , Methamphetamine , Morphine , N-des-tapentadol , Naloxone , Norbuprenorphine , Nordiazepam , Norfentanyl , Norhydrocodone , Norketamine , Normeperidine , Nortryptiline , Opiates , Opioid , Oxazepam , Oxycodone , Oxymorphone , Pain Management , Phencyclidine , Phenobarbital , Posters , Pregabalin , Ritalinic acid , Secobarbital , Tapentadol , Temazepam , Tramadol , Urine , Zolpidem , Zolpidem-phenyl-4-COOH , z-drugs , α-OH alprazolam
The major metabolites identified in the urine after topical administration are salicyluric acid (52%), salicylate glucuronides (42%) and free Salicylic Acid (6%). The urinary metabolites after percutaneous absorption differ from those after oral salicylate administration; those derived from percutaneous absorption contain more salicylate glucuronides and less salicyluric and Salicylic Acid. Almost 95% of a single dose of salicylate is excreted within 24 hours of its entrance into the extracellular space. Fifty to eighty percent of salicylate is protein bound to albumin. Salicylates compete with the binding of several drugs and can modify the action of these drugs. By similar competitive mechanisms other drugs can influence the serum levels of salicylate. (See PRECAUTIONS.)